Journal:

Article Title: Lack of Complement Inhibitors in the Outer Intracranial Artery Aneurysm Wall Associates with Complement Terminal Pathway Activation

doi: 10.2353/ajpath.2010.091172

Figure Lengend Snippet: Primary Antibodies Used in Immunohistochemical and Immunofluorescence Experiments ∗

Article Snippet: For negative controls, the primary antibody was omitted or replaced with an irrelevant antibody of the same immunoglobulin subtype as the primary antibody. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Antibody target (Clone) Role Type Dilution Source † n ‡ Double stained with Factor H Soluble AP inhibitor Goat pAb 1:400 Calbiochem 10 TCC C4b binding protein Soluble decay accelerator of C4b2a; CP inhibition Rabbit pAb 1:1000 (37) 35 — C4b binding protein Soluble decay accelerator of C4b2a; CP inhibition Sheep pAb 1:100 The Binding Site 7 TCC Protein S Anticoagulant protein; in complex with C4bp Rabbit pAb 1:400 Sigma 18 — Membrane cofactor protein/CD46 (J4-48) Membrane cofactor for C4b and C3b cleavage; CP and AP inhibition Mouse mAb 1:200 Immunotech 13 — Decay accelerating factor/CD55 (BRIC 216) Membrane decay accelerator of C4b2a and C3bBb; CP and AP inhibition Mouse mAb 1:200 IBGRL 13 — Clusterin Soluble TP inhibitor Mouse mAb 1:100 Quidel 13 — Vitronectin/S-protein Soluble TP inhibitor Mouse mAb 1:100 Quidel 13 — Protectin/CD59 (BRIC 229) Membrane-bound C5b-9 inhibitor; inhibition of MAC Mouse mAb 1:200 IBGRL 44 TCC Protectin/CD59 Membrane-bound C5b-9 inhibitor; inhibition of MAC Goat pAb 1:50 (38) 12 TCC C5b-9 (A239) Terminal complement complex Mouse mAb 1:150 Quidel 44 Factor H, CD59 C5b-9 (aE11) Terminal complement complex Mouse mAb 1:200 Dako 13 C4bp, CD59 Open in a separate window * CP, complement classical pathway; AP, complement alternative pathway; TP, complement terminal pathway; MAC, membrane attack complex; pAb, polyclonal antibody; mAb, monoclonal antibody; TCC, terminal complement complex; C4bp, C4b binding protein.

Techniques: Immunohistochemical staining, Immunofluorescence, Staining, Binding Assay, Inhibition, Membrane